Journal: Marine Drugs

Article Title: Multifunctional Cell Regulation Activities of the Mussel Lectin SeviL: Induction of Macrophage Polarization toward the M1 Functional Phenotype

doi: 10.3390/md22060269

Figure Lengend Snippet: Effect of SeviL on RAW264.7 cells induced the secretion of cytokines. Cells were cultured without or with various concentrations of SeviL (0–10 µg/mL) for 24 h in the presence or absence of 10 mM of lactose, the haptenic sugar of SeviL. Cytokine, IL-6 ( A ), and TNF-α ( B ) levels in the supernatant were measured via ELISA. The data shown are means ± SE ( n = 3). Compared with the control group, * p < 0.05.

Article Snippet: The levels of IL-6 and TNF-α in cell culture medium (mouse IL-6 ELISA Kit, Proteintech, Japan and mouse TNF-α ELISA Kit, Fujifilm Wako chemicals, Tokyo, Japan) were measured via ELISA according to the manufacturer’s instructions.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Control